Anti-Ghrelin O acyltransferase antibody (ab115036)

Overview

  • Product name
    Anti-Ghrelin O acyltransferase antibody
    See all Ghrelin O acyltransferase primary antibodies
  • Description
    Goat polyclonal to Ghrelin O acyltransferase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide:

    CHLGLHYTEYYLGEP

    , corresponding to internal sequence amino acids 97-111 of Mouse MBOAT4 (NP_001119786.1.)

  • Positive control
    • Mouse Pancreas lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab115036 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
  • Tissue specificity
    Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.
  • Sequence similarities
    Belongs to the membrane-bound acyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKSG89 antibody
    • ghrelin o transferas antibody
    • Ghrelin O-acyltransferase antibody
    • GOAT antibody
    • MBOA4_HUMAN antibody
    • Mboat4 antibody
    • Membrane-bound O-acyltransferase domain-containing protein 4 antibody
    • O-acyltransferase (membrane bound) domain containing 4 antibody
    • O-acyltransferase domain-containing protein 4 antibody
    • OACT4 antibody
    see all

Images

  • Anti-Ghrelin O acyltransferase antibody (ab115036) at 0.5 µg/ml + Mouse Pancreas lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References

ab115036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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