Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99449 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 23879975

Target

  • Function
    Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
  • Tissue specificity
    Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.
  • Sequence similarities
    Belongs to the membrane-bound acyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKSG89 antibody
    • ghrelin o transferas antibody
    • Ghrelin O-acyltransferase antibody
    • GOAT antibody
    • MBOA4_HUMAN antibody
    • Mboat4 antibody
    • Membrane-bound O-acyltransferase domain-containing protein 4 antibody
    • O-acyltransferase (membrane bound) domain containing 4 antibody
    • O-acyltransferase domain-containing protein 4 antibody
    • OACT4 antibody
    see all

Images

  • ab99449 staining Ghrelin O acyltransferase in Human Prostate cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100 in blocking buffer) for overnight at 4°C. An HRP-conjugated secondary was used and shows diffuse cytoplasmic staining.

References

This product has been referenced in:
  • Seim I  et al. Ghrelin O-acyltransferase (GOAT) is expressed in prostate cancer tissues and cell lines and expression is differentially regulated in vitro by ghrelin. Reprod Biol Endocrinol 11:70 (2013). IHC-P ; Human . Read more (PubMed: 23879975) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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