Overview

  • Product name
    Anti-Ghrelin Receptor antibody
    See all Ghrelin Receptor primary antibodies
  • Description
    Rabbit polyclonal to Ghrelin Receptor
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Rat) from near the N-terminal of Ghrelin receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab85104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 40 kDa.Can be blocked with Ghrelin Receptor peptide (ab134515).
IHC-P 1/100 - 1/500.

Target

  • Function
    Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).
  • Tissue specificity
    Pituitary and hypothalamus.
  • Involvement in disease
    Defects in GHSR may be a cause of idiopathic short stature autosomal (ISSA) [MIM:604271]. Short stature is defined by a subnormal rate of growth.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GH releasing peptide receptor antibody
    • GH-releasing peptide receptor antibody
    • Ghrelin receptor antibody
    • GHRP antibody
    • GHRPR antibody
    • GHS-R antibody
    • GHSR antibody
    • GHSR_HUMAN antibody
    • Growth hormone secretagogue receptor 1 antibody
    • Growth hormone secretagogue receptor type 1 antibody
    • growth hormone secretatgogue receptor antibody
    see all

Images

  • ab85104 at 1/200 dilution staining Ghrelin Receptors in human fetal brain tissue section by  immunohistochemistry (Formalin/ PFA fixed, paraffin-embedded tissue sections).

  • Rat liver lysate, 1:500 dilution.

References

This product has been referenced in:
  • Merigo F  et al. Molecules implicated in glucose homeostasis are differentially expressed in the trachea of lean and obese Zucker rats. Eur J Histochem 60:2557 (2016). Read more (PubMed: 26972710) »
  • Galvão A  et al. Opposing roles of leptin and ghrelin in the equine corpus luteum regulation: an in vitro study. Mediators Inflamm 2014:682193 (2014). Read more (PubMed: 25125800) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (8% acrylamide)
Sample
Mouse Tissue lysate - whole (whole brain and Pancreas)
Specification
whole brain and Pancreas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Jeffrey Gagnon

Verified customer

Submitted Nov 12 2013

Antibody ab92520 is raised against a ghrelin receptor amino acid sequence that is found in both isoforms of the human receptor, so it should be capable of recognizing both. Antibody ab85104 is raised against a sequence derived from rat receptor that is...

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The isoforms 1A and 1B differ in amino acid 266-289 and 290-366, as these are missing in Isoform 1B. http://www.uniprot.org/uniprot/Q92847. Based on this data antibody ab125457 would detect the Isoform 1A only.

ab85104, ab14480, ab14481,...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acd
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 28 2013

Thank you for your reply.
Unfortunately, we haven't tested if this ab85104 Anti-Ghrelin Receptor antibody can distinguish between the GHS-R1a and GHS-R1b protein. However, as the immunogen is derived from the conserved N-terminus of both forms, it...

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Thanks for the purchase order information.
I am currently processing your replacement order which you should receive next week.
Thanks for your patience. Please contact me if you have any further questions.

I apologize for the confusion.
I just wanted to confirm that you were testing reactivity of the antibody with protein based on the rat or human sequence. As you confirmed you are working with human protein it does appear there is an issue with the...

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Thanks for the additional information.
I just want to confirm that your cDNA for GHSR-1 is based on human or rat sequence, as those are the tested species to date.
Thanks again!

Thank you for submitting your completed questionnaire.
Just a few additional questions:
1) Is a full length GHSR-1 being expressed or is the consturct a fragment of GHSR-1?
2) What species of cDNA was used to produce the GHSR-1 clone? Read More

Application
Western blot
Sample
Mouse Cell lysate - whole cell (STC1)
Loading amount
20 µg
Specification
STC1
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

Submitted Oct 28 2010

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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