Overview

  • Product nameAnti-GHRH antibody
    See all GHRH primary antibodies
  • Description
    Rabbit polyclonal to GHRH
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GHRH aa 1-40.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
IHC-Fr 1/200 - 1/800. (See Bloch et al 1986 reference).
RIA Use at an assay dependent concentration. (See Bloch et al 1986 reference).

Target

  • FunctionGRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
  • Sequence similaritiesBelongs to the glucagon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GHRF antibody
    • GHRH antibody
    • GRF antibody
    • Growth hormone releasing factor antibody
    • Growth Hormone Releasing Hormone antibody
    • Growth hormone-releasing factor antibody
    • Growth hormone-releasing hormone antibody
    • INN antibody
    • MGC119781 antibody
    • OTTHUMP00000030915 antibody
    • Sermorelin antibody
    • SLIB_HUMAN antibody
    • Somatocrinin antibody
    • Somatoliberin antibody
    • Somatorelin antibody
    see all

Anti-GHRH antibody images

  • ICC/IF image of ab8899 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8899, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GHRH antibody (ab8899)

This product has been referenced in:
  • Rick FG  et al. Antagonists of growth hormone-releasing hormone inhibit growth of androgen-independent prostate cancer through inactivation of ERK and Akt kinases. Proc Natl Acad Sci U S A 109:1655-60 (2012). WB ; Human . Read more (PubMed: 22307626) »

See 1 Publication for this product

Product Wall

The molecular weight of the antibody is approximately 160 kDa. It consists of two heavy chains, which are about 53 kDa, and two light chains, which are 25 kDa.

The protein the antibody recognizes, GHRH, is much smaller. The precursor is 12.4...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"