Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)

Overview

  • Product name
    Anti-Giantin antibody [9B6] - Golgi Marker
    See all Giantin primary antibodies
  • Description
    Mouse monoclonal [9B6] to Giantin - Golgi Marker
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein of Rat Giantin.

  • Positive control
    • HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    9B6
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function
    May participate in forming intercisternal cross-bridges of the Golgi complex.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 372 kDa Golgi complex associated protein antibody
    • 372 kDa Golgi complex-associated protein antibody
    • GCP antibody
    • GCP372 antibody
    • Giantin antibody
    • GOGB1_HUMAN antibody
    • GOLGB1 antibody
    • Golgi autoantigen golgin subfamily a member 1 antibody
    • Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody
    • Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody
    • Golgi complex associated protein 372kDa antibody
    • Golgi complex-associated protein 372kDa antibody
    • Golgin B1 antibody
    • Golgin B1, golgi integral membrane protein antibody
    • Golgin subfamily B member 1 antibody
    • GOLIM1 antibody
    • Macrogolgin antibody
    • MGC33154 antibody
    see all

Anti-Giantin antibody [9B6] - Golgi Marker images

  •  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) and (ab37266, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green LCS1 (ab138904) was used to stain the cell nuclei (green) at a dilution of 1/500.

     

  • ICC/IF image of ab37266 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab37266, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab37266 (1/200) staining Giantin in assynchronous HeLa cells (green). Cells were fixed in Methanol and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to Abreview for further experimental details.

    See Abreview

  • ICC/IF image of ab37266 stained Hek293 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)

This product has been referenced in:
  • Starheim KK  et al. Depletion of the human N-terminal acetyltransferase hNaa30 disrupts Golgi integrity and ARFRP1 localization. Biosci Rep 37:N/A (2017). ICC/IF ; Human . Read more (PubMed: 28356483) »
  • Zollo A  et al. Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer's Disease Patients Carrying the APOE Epsilon 4 Allele. Neural Plast 2017:1892612 (2017). ICC/IF ; Human . Read more (PubMed: 28634550) »

See all 24 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Muscle cell line (C2C12))
Permeabilization
Yes - Triton in PBS
Specification
Muscle cell line (C2C12)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1000µg/mL · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (A549)
Specification
A549
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Rat Cell (Rat hepatocytes)
Specification
Rat hepatocytes
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Caco-2 cell)
Specification
Caco-2 cell
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 27 2013

Thank you for your reply.

For paraffin-embedded tissue sections (IHC-P), we recommend Human Tissue sections (renal cancer (Type II papillary)).

http://www.abcam.com/Giantin-antibody-9B6-Golgi-Marker-ab37266-reviews.html?intabreviewi...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human cervic cancer)
Specification
human cervic cancer
Fixative
Methanol
Permeabilization
Yes - 0.1% triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 04 2012

I am very pleased to hear you would like to accept our offer and test ab37266 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hTERT-RPE1)
Specification
hTERT-RPE1
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 07 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, U2OS, 293T)
Loading amount
30 µg
Specification
HeLa, U2OS, 293T
Gel Running Conditions
Reduced Denaturing (5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jul 30 2008

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