Overview

  • Product nameAnti-Giantin antibody
    See all Giantin primary antibodies
  • Description
    Rabbit polyclonal to Giantin
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (NKYIEEMKAQ GGTVLPTEPQ SEEQLSKHDK SSTEEEMEIE KIKHKLQEKE) of Human Giantin (NP_004478).

  • Positive control
    • 721_B cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 376 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay participate in forming intercisternal cross-bridges of the Golgi complex.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 372 kDa Golgi complex associated protein antibody
    • 372 kDa Golgi complex-associated protein antibody
    • GCP antibody
    • GCP372 antibody
    • Giantin antibody
    • GOGB1_HUMAN antibody
    • GOLGB1 antibody
    • Golgi autoantigen golgin subfamily a member 1 antibody
    • Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody
    • Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody
    • Golgi complex associated protein 372kDa antibody
    • Golgi complex-associated protein 372kDa antibody
    • Golgin B1 antibody
    • Golgin B1, golgi integral membrane protein antibody
    • Golgin subfamily B member 1 antibody
    • GOLIM1 antibody
    • Macrogolgin antibody
    • MGC33154 antibody
    see all

Anti-Giantin antibody images

  • Anti-Giantin antibody (ab93281) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 376 kDa
    Gel concentration 6-18%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling Giantin with ab93281 at 5µg/ml.

References for Anti-Giantin antibody (ab93281)

ab93281 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 17 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (LNCaP - prostate cancer cells)
Permeabilization Yes - 0.2% Triton X-100
Specification LNCaP - prostate cancer cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jun 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"