Anti-GIGYF1 antibody (ab121784)
Key features and details
- Rabbit polyclonal to GIGYF1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GIGYF1 antibody
See all GIGYF1 primary antibodies -
Description
Rabbit polyclonal to GIGYF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 402-499 (GIQLSPGVGS SAGPPGDLED DEGLKHLQQE AEKLVASLQD SSLEEEQFTA AMQTQGLRHS AAATALPLSH GAARKWFYKD PQGEIQGPFT TQEMAEWF) of Human GIGYF1.
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Positive control
- Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1. -
Sequence similarities
Belongs to the PERQ family.
Contains 1 GYF domain. -
Domain
The GYF domain interacts with GRB10. - Information by UniProt
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Database links
- Entrez Gene: 64599 Human
- Omim: 612064 Human
- SwissProt: O75420 Human
- Unigene: 414396 Human
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Alternative names
- CDS2 antibody
- GIGYF1 antibody
- GRB10-interacting GYF protein 1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab121784 has been referenced in 2 publications.
- Nordgaard C et al. Regulation of the Golgi Apparatus by p38 and JNK Kinases during Cellular Stress Responses. Int J Mol Sci 22:N/A (2021). PubMed: 34502507
- Tollenaere MAX et al. GIGYF1/2-Driven Cooperation between ZNF598 and TTP in Posttranscriptional Regulation of Inflammatory Signaling. Cell Rep 26:3511-3521.e4 (2019). PubMed: 30917308