Overview

  • Product name
  • Description
    Rabbit polyclonal to GIGYF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human GIGYF1 aa 350-400. The exact sequence is proprietary. (NP_072096.2)
    Sequence:

    E AGGKELTPLP PQEEKSSSPS PLPTLGPLWG TNGDGDETAE KEPPAAEDDI


    Database link: O75420

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 - 8.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab177509 was affinity purified using an epitope specific to GIGYF1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 115 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1.
  • Sequence similarities
    Belongs to the PERQ family.
    Contains 1 GYF domain.
  • Domain
    The GYF domain interacts with GRB10.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDS2 antibody
    • GIGYF1 antibody
    • GRB10-interacting GYF protein 1 antibody
    • GYF1 antibody
    • PERQ amino acid-rich with GYF domain-containing protein 1 antibody
    • PERQ1 antibody
    • PERQ1_HUMAN antibody
    • Postmeiotic segregation increased 2-like 12 antibody
    • PP3360 antibody
    see all

Images

  • All lanes : Anti-GIGYF1 antibody (ab177509) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 115 kDa


    Exposure time: 10 seconds
  • Detection of GIGYF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177509 at 6 µg/mg lysate for IP (Lane 1). For WB detection,  ab177509 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab177509 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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