Overview

  • Product nameAnti-GilZ / TilZ antibody
    See all GilZ / TilZ primary antibodies
  • Description
    Mouse monoclonal to GilZ / TilZ
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cyt, CHIPseqmore details
  • Species reactivity
    Reacts with: Mouse, Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: MAQSKLDCRS PVGLDCCNCC LDLAHRSGLQ RGSSGENNNP GSPTVSNFRQ LQEKLVFENL NTDKLNSIMR QDSLEPVLRD PCYLINEGIC NRNIDQT, corresponding to amino acids 1-98 of Human GilZ/ TilZ

Properties

Applications

Our Abpromise guarantee covers the use of ab55015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
CHIPseq Use at an assay dependent concentration. PubMed: 23467089

Target

  • FunctionProtects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities.
  • Tissue specificityExpressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells.
  • Sequence similaritiesBelongs to the TSC-22/Dip/Bun family.
  • DomainThe leucine-zipper is involved in homodimerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta sleep-inducing peptide immunoreactor antibody
    • DIP antibody
    • DSIP immunoreactive leucine zipper protein antibody
    • DSIP-immunoreactive peptide antibody
    • Dsip1 antibody
    • Dsipi antibody
    • GILZ antibody
    • Glucocorticoid-induced leucine zipper protein antibody
    • hDIP antibody
    • Protein DIP antibody
    • T22D3_HUMAN antibody
    • TSC-22-like protein antibody
    • TSC-22-related protein antibody
    • TSC-22R antibody
    • TSC22 domain family protein 3 antibody
    • TSC22 domain family, member 3 antibody
    • TSC22 related inducible leucine zipper 3 antibody
    • TSC22D3 antibody
    see all

Anti-GilZ / TilZ antibody images

  • Overlay histogram showing HeLa cells stained with ab55015 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55015, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • GilZ / TilZ antibody (ab55015) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
  • Western blot against tagged recombinant protein immunogen using ab55015 GilZ / TilZ antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab55015 at 10 ug/ml staining GilZ / TilZ in human Hela cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-GilZ / TilZ antibody (ab55015)

This product has been referenced in:
  • Mao Y  et al. Milatuzumab-conjugated liposomes as targeted dexamethasone carriers for therapeutic delivery in CD74+ B-cell malignancies. Clin Cancer Res 19:347-56 (2013). WB . Read more (PubMed: 23209030) »
  • Yosef N  et al. Dynamic regulatory network controlling TH17 cell differentiation. Nature 496:461-8 (2013). CHIPseq ; Mouse . Read more (PubMed: 23467089) »

See all 3 Publications for this product

Product Wall

Merci de votre intérêt pour ab130301. A ma connaissance, cet anticorps n’a pas encore été testé en ELISA et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminai...

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