Overview

  • Product nameAnti-GIP antibody [04]
    See all GIP primary antibodies
  • Description
    Mouse monoclonal [04] to GIP
  • SpecificityAb30679 recognises GIP. It cross reacts up to 10% with PACAP and GLP2 but <0.1% with glucagon, GLP1 or VIP.
  • Tested applicationsSuitable for: IHC-P, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic human GIP coupled to carrier. The immunogen was adsorbed onto Al(OH)3 and emulsified in Freund's incomplete adjuvant.

  • General notes


    Fusion partner: SP2/mIL6.

Properties

Applications

Our Abpromise guarantee covers the use of ab30679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/10000.

This dilution guideline applies to glutaraldehyde linked GIP OA (molar ratio 4:1) coated at 1 mg/mL. With GIP coated directly at 2 µg/mL the dilution guideline is 1/2000.

IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionPotent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • Sequence similaritiesBelongs to the glucagon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastric Inhibitory Peptide antibody
    • Gastric inhibitory polypeptide antibody
    • Gastric inhibitory polypeptide precursor antibody
    • GIP antibody
    • GIP_HUMAN antibody
    • Glucose dependent insulinotropic polypeptide antibody
    • Glucose-dependent insulinotropic polypeptide antibody
    • Incretin hormone antibody
    see all

Anti-GIP antibody [04] images

  • ab30679 at 1/100 staining GIP in human duodenum by IHC-P.

  • ab30679 at 1/100 staining GIP in human small intestine by IHC-P.

References for Anti-GIP antibody [04] (ab30679)

This product has been referenced in:
  • Young RL  et al. Disordered control of intestinal sweet taste receptor expression and glucose absorption in type 2 diabetes. Diabetes 62:3532-41 (2013). Human . Read more (PubMed: 23761104) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (gut)
Specification gut
Fixative Paraformaldehyde
Permeabilization Yes - Antigen retrieval
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Duodenum)
Specification Duodenum
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Goat Sera as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Ms. Bridgette Chia

Verified customer

Submitted Aug 10 2010

Thank you for your enquiry regarding our product ab30679. I am sorry you have had difficulty obtaining satisfactory results from your binding experiments with this antibody. Although this antibody has been tested by ELISA, it has not been tested for th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"