Overview

  • Product nameAnti-GIP antibody
    See all GIP primary antibodies
  • Description
    Rabbit polyclonal to GIP
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GIP.

  • Positive control
    • This antibody gave a positive signal in Human Pancreas tissue lysate within western blot and human pancreas FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab72167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPotent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • Sequence similaritiesBelongs to the glucagon family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastric Inhibitory Peptide antibody
    • Gastric inhibitory polypeptide antibody
    • Gastric inhibitory polypeptide precursor antibody
    • GIP antibody
    • GIP_HUMAN antibody
    • Glucose dependent insulinotropic polypeptide antibody
    • Glucose-dependent insulinotropic polypeptide antibody
    • Incretin hormone antibody
    see all

Anti-GIP antibody images

  • Anti-GIP antibody (ab72167) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • IHC image of ab72167 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72167, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-GIP antibody (ab72167)

ab72167 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72167.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"