The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human GIPC1 peptide (ab23130).
Use at an assay dependent dilution. PubMed: 20634288
RelevanceGIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling.
Cellular localizationCell Membrane, peripheral membrane protein and Cytoplasmic.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GIPC1 antibody (ab5951)This image is courtesy of an anonymous Abreview
ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.
ICC/IF image of ab5951 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5951, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GIPC1 antibody (ab5951)
This product has been referenced in:
Wu D et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem285:28643-50 (2010).
Read more (PubMed: 20634288) »
Valdembri D et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol7:e25 (2009).
Read more (PubMed: 19175293) »