Overview

  • Product nameAnti-GIPC1 antibody
    See all GIPC1 primary antibodies
  • Description
    Goat polyclonal to GIPC1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1.

  • Positive control
    • HeLa Lysate.
  • General notesGenBank Accession Number – NP_005707

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab5951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human GIPC1 peptide (ab23130).
ICC/IF Use at an assay dependent dilution. PubMed: 20634288

Target

  • RelevanceGIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling.
  • Cellular localizationCell Membrane, peripheral membrane protein and Cytoplasmic.
  • Database links
  • Alternative names
    • C19ORF3 antibody
    • Chromosome 19 open reading frame 3 antibody
    • GAIP C-terminus-interacting protein antibody
    • GIPC antibody
    • GIPC PDZ domain containing family member 1 antibody
    • GLUT1 C terminal binding protein antibody
    • GLUT1CBP antibody
    • Hs.6454 antibody
    • IGF 1 receptor interacting protein 1 antibody
    • IIP 1 antibody
    • IIP1 antibody
    • NIP antibody
    • PDZ domain-containing protein GIPC1 antibody
    • Regulator of G protein signalling 19 interacting protein 1 antibody
    • RGS-GAIP-interacting protein antibody
    • RGS19 interacting protein 1 antibody
    • RGS19IP1 antibody
    • SEMCAP antibody
    • SYNECTIIN antibody
    • Tax interaction protein 2 antibody
    • TIP 2 antibody
    see all

Anti-GIPC1 antibody images



  • Predicted band size : 38 kDa


    ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
  • Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size : 38 kDa
    Observed band size : 24,38 kDa (why is the actual band size different from the predicted?)
  • ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.

    See Abreview

  • ICC/IF image of ab5951 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5951, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GIPC1 antibody (ab5951)

This product has been referenced in:
  • Wu D  et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF ; Human . Read more (PubMed: 20634288) »
  • Valdembri D  et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB ; Human . Read more (PubMed: 19175293) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (ventral striatum)
Gel Running Conditions Non-reduced Denaturing (10% TGX)
Loading amount 30 µg
Specification ventral striatum
Blocking step licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (prostate tissue section)
Specification prostate tissue section
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Arterial endothelial cells)
Loading amount 30 µg
Specification Arterial endothelial cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Elena Astanina

Verified customer

Submitted Apr 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"