Recombinant
RabMAb

Anti-Girdin (phospho Y1765) antibody [SP158] (ab130424)

Overview

  • Product name
    Anti-Girdin (phospho Y1765) antibody [SP158]
    See all Girdin primary antibodies
  • Description
    Rabbit monoclonal [SP158] to Girdin (phospho Y1765)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Girdin (phospho Y1765).

  • Positive control
    • IHC-P: Human colon adenocarcinoma, colon, breast ductal carcinoma, bladder transitional cell carcinoma, bladder and skin tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab130424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Target

  • Function
    Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.
  • Tissue specificity
    Expressed ubiquitously.
  • Sequence similarities
    Belongs to the CCDC88 family.
  • Post-translational
    modifications
    Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.
  • Cellular localization
    Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKT iphosphorylation enhancer antibody
    • Akt phosphorylation enhancer antibody
    • APE antibody
    • Ccdc88a antibody
    • Coiled coil domain containing 88A antibody
    • Coiled coil domain containing protein 88A antibody
    • Coiled-coil domain-containing protein 88A antibody
    • FLJ10392 antibody
    • G alpha interacting vesicle associated protein antibody
    • G alpha-interacting vesicle-associated protein antibody
    • Galpha interacting vesicle associated protein antibody
    • Girders of actin filament antibody
    • Girdin antibody
    • GIV antibody
    • GRDN antibody
    • GRDN_HUMAN antibody
    • HkRP1 antibody
    • Hook related protein 1 antibody
    • Hook-related protein 1 antibody
    • KIAA1212 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma Stage 4 tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma Stage 3 tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma Stage 2 tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder transitional carcinoma tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Girdin (phospho Y1765) using ab130424 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Lopez-Sanchez I  et al. Focal adhesions are foci for tyrosine-based signal transduction via GIV/Girdin and G proteins. Mol Biol Cell 26:4313-24 (2015). Read more (PubMed: 26446841) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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