Overview

  • Product name
  • Description
    Rabbit polyclonal to GIT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within internal amino acids 375-631 of Human GIT1 (Uniprot ID Q9Y2X7).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells; Human mixed OVCA tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 84 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
  • Sequence similarities
    Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
  • Domain
    The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
  • Cellular localization
    Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARF GAP GIT1 antibody
    • ARF GTPase activating protein GIT1 antibody
    • ARF GTPase-activating protein GIT1 antibody
    • CAT 1 antibody
    • CAT-1 antibody
    • CaT1 antibody
    • Cool associated and tyrosine phosphorylated protein 1 antibody
    • Cool-associated and tyrosine-phosphorylated protein 1 antibody
    • G protein coupled receptor kinase interacting ArfGAP 1 antibody
    • G protein coupled receptor kinase interactor 1 antibody
    • G protein-coupled receptor kinase-interactor 1 antibody
    • GIT1 antibody
    • GIT1_HUMAN antibody
    • GRK interacting protein 1 antibody
    • GRK-interacting protein 1 antibody
    see all

Images

  • Anti-GIT1 antibody (ab153958) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 84 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa, labeling GIT1 with ab153958 at 1/500 dilution. Blue: Costaining with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human mixed OVCA tissue, labeling GIT1 with ab153958 at 1/100 dilution.

References

ab153958 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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