• Product nameAnti-GIT1 antibody
    See all GIT1 primary antibodies
  • Description
    Rabbit polyclonal to GIT1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues surrounding Y510 of human GIT1, conjugated to KLH.

  • Positive control
    • mouse cerebellum tissue lysate



Our Abpromise guarantee covers the use of ab75925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 84 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.


  • FunctionGTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
  • DomainThe paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
  • Post-translational
    Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
  • Cellular localizationCytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARF GAP GIT1 antibody
    • ARF GTPase activating protein GIT1 antibody
    • ARF GTPase-activating protein GIT1 antibody
    • CAT 1 antibody
    • CAT-1 antibody
    • CaT1 antibody
    • Cool associated and tyrosine phosphorylated protein 1 antibody
    • Cool-associated and tyrosine-phosphorylated protein 1 antibody
    • G protein coupled receptor kinase interacting ArfGAP 1 antibody
    • G protein coupled receptor kinase interactor 1 antibody
    • G protein-coupled receptor kinase-interactor 1 antibody
    • GIT1 antibody
    • GIT1_HUMAN antibody
    • GRK interacting protein 1 antibody
    • GRK-interacting protein 1 antibody
    see all

Anti-GIT1 antibody images

  • Anti-GIT1 antibody (ab75925) at 1/50 dilution + mouse cerebellum tissue lysate at 35 µg

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.
  • ab75925 at 1/50 dilution staining GIT1 in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-GIT1 antibody (ab75925)

ab75925 has not yet been referenced specifically in any publications.

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