Overview

  • Product nameAnti-GIT1 antibody
    See all GIT1 primary antibodies
  • Description
    Rabbit polyclonal to GIT1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 375 and 425 of Human GIT1 (NP_054749.2).

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionGTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
  • Sequence similaritiesContains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
  • DomainThe paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
  • Cellular localizationCytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARF GAP GIT1 antibody
    • ARF GTPase activating protein GIT1 antibody
    • ARF GTPase-activating protein GIT1 antibody
    • CAT 1 antibody
    • CAT-1 antibody
    • CaT1 antibody
    • Cool associated and tyrosine phosphorylated protein 1 antibody
    • Cool-associated and tyrosine-phosphorylated protein 1 antibody
    • G protein coupled receptor kinase interacting ArfGAP 1 antibody
    • G protein coupled receptor kinase interactor 1 antibody
    • G protein-coupled receptor kinase-interactor 1 antibody
    • GIT1 antibody
    • GIT1_HUMAN antibody
    • GRK interacting protein 1 antibody
    • GRK-interacting protein 1 antibody
    see all

Anti-GIT1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling GIT1 with ab86235 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-GIT1 antibody (ab86235) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 84 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 275 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of GIT1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86235 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-GIT1 antibody (ab86235)

ab86235 has not yet been referenced specifically in any publications.

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