Recombinant
RabMAb

Anti-GIT1 antibody [EPR10367] (ab156001)

Overview

  • Product name
    Anti-GIT1 antibody [EPR10367]
    See all GIT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10367] to GIT1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GIT1 (UniProt Q9Y2X7).

  • Positive control
    • HeLa, 293T and fetal brain lysates; 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab156001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
    • Sequence similarities
      Contains 3 ANK repeats.
      Contains 1 Arf-GAP domain.
    • Domain
      The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
    • Cellular localization
      Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARF GAP GIT1 antibody
      • ARF GTPase activating protein GIT1 antibody
      • ARF GTPase-activating protein GIT1 antibody
      • CAT 1 antibody
      • CAT-1 antibody
      • CaT1 antibody
      • Cool associated and tyrosine phosphorylated protein 1 antibody
      • Cool-associated and tyrosine-phosphorylated protein 1 antibody
      • G protein coupled receptor kinase interacting ArfGAP 1 antibody
      • G protein coupled receptor kinase interactor 1 antibody
      • G protein-coupled receptor kinase-interactor 1 antibody
      • GIT1 antibody
      • GIT1_HUMAN antibody
      • GRK interacting protein 1 antibody
      • GRK-interacting protein 1 antibody
      see all

    Images

    • All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 84 kDa
    • Flow cytometric analysis of permeabilized 293T cells labeling GIT1 with ab156001 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:
    • Sulimenko V  et al. Microtubule nucleation in mouse bone marrow-derived mast cells is regulated by the concerted action of GIT1/ßPIX proteins and calcium. J Immunol 194:4099-111 (2015). Read more (PubMed: 25821222) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (3T3 cells)
    Permeabilization
    Yes - triton X100
    Specification
    3T3 cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Peter Gould

    Verified customer

    Submitted Nov 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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