Overview

  • Product name
  • Description
    Goat polyclonal to GJB2
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    YLLIRYCSGKSKKP

    , corresponding to C terminal amino acids 212-225 of Human GJB2

  • Positive control
    • Mouse Brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration. PubMed: 21536677
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in disease
    Defects in GJB2 are the cause of deafness autosomal recessive type 1A (DFNB1A) [MIM:220290]. DFNB1A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in GJB2 are the cause of deafness autosomal dominant type 3A (DFNA3A) [MIM:601544].
    Defects in GJB2 are a cause of Vohwinkel syndrome (VS) [MIM:124500]. VS is an autosomal dominant disease characterized by hyperkeratosis, constriction on finger and toes and congenital deafness.
    Defects in GJB2 are a cause of palmoplantar keratoderma with deafness (PPKDFN) [MIM:148350]. PPKDFN is an autosomal dominant disorder characterized by the association of palmoplantar hyperkeratosis with progressive, bilateral, high-frequency, sensorineural deafness.
    Defects in GJB2 are a cause of keratitis-ichthyosis-deafness syndrome (KID syndrome) [MIM:148210]; an autosomal dominant form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. KID syndrome is characterized by the association of hyperkeratotic skin lesions with vascularizing keratitis and profound sensorineural hearing loss. Clinical features include deafness, ichthyosis, photobia, absent or decreased eyebrows, sparse or absent scalp hair, decreased sweating and dysplastic finger and toenails.
    Defects in GJB2 are the cause of Bart-Pumphrey syndrome (BPS) [MIM:149200]. BPS is an autosomal dominant disorder characterized by sensorineural hearing loss, palmoplantar keratoderma, knuckle pads, and leukonychia, It shows considerable phenotypic variability.
    Defects in GJB2 are the cause of ichthyosis hystrix-like with deafness syndrome (HID syndrome) [MIM:602540]. HID syndrome is an autosomal-dominant inherited keratinizing disorder characterized by sensorineural deafness and spiky hyperkeratosis affecting the entire skin. HID syndrome is considered to differ from the similar KID syndrome in the extent and time of occurrence of skin symptoms and the severity of the associated keratitis.
  • Sequence similarities
    Belongs to the connexin family. Beta-type (group I) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • connexin 26 antibody
    • Connexin-26 antibody
    • Cx26 antibody
    • CXB2_HUMAN antibody
    • DFNA3 antibody
    • DFNB1 antibody
    • Gap junction beta-2 protein antibody
    • GJB2 antibody
    • HID antibody
    • KID antibody
    • NSRD1 antibody
    • PPK antibody
    see all

Images

  • All lanes : Anti-GJB2 antibody (ab59020) at 1/5000 dilution

    Lane 1 : MCF-7 cells
    Lane 2 : HCC1806 cells

    Secondary
    HRP-conjugated rabbit anti-goat IgG polyclonal at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa


    Exposure time : 5 seconds

    This image is courtesy of an Abreview submitted by Abhijith Shettar

    Blocked with 5% milk for 1 hour at 25°C.

    See Abreview

  • Anti-GJB2 antibody (ab59020) at 0.01 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer)

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:
  • Fort AG  et al. In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors. J Biol Chem 286:22875-85 (2011). ICC/IF ; Rat . Read more (PubMed: 21536677) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Human breast cancer cell lines)
Specification
Human breast cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Abhijith Shettar

Verified customer

Submitted Jun 17 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Protein Block, Serum-Free - Dako as blocking agent for 30 minute(s) · Concentration: 0.25% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2013

Thank you for contacting us with your question. We would recommend starting with 5 ug/mL in IF, which would be a 1:100 dilution, though this concentration will need to be intrinsically optimized for your samples. I hope this information ...

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