Overview

  • Product nameAnti-GK2 antibody
    See all GK2 primary antibodies
  • Description
    Rabbit polyclonal to GK2
  • Specificityab65225 detects endogenous levels of total GK2 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human GK2

  • Positive control
    • Extracts from HeLa, 293 and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Target

  • RelevanceGK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.
  • Cellular localizationMitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.
  • Database links
  • Alternative names
    • ATP:glycerol 3 phosphotransferase antibody
    • EC 2.7.1.30 antibody
    • GK 2 antibody
    • GK antibody
    • GKP2 antibody
    • GKTA antibody
    • Glycerokinase antibody
    • Glycerol kinase 2 antibody
    • Glycerol kinase testis specific 2 antibody
    see all

Anti-GK2 antibody images

  • All lanes : Anti-GK2 antibody (ab65225) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : 293 cell extract
    Lane 3 : Jurkat cell extract
    Lane 4 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GK2 antibody (ab65225)

ab65225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"