Overview

  • Product name
  • Description
    Rabbit polyclonal to GK2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 117 and 308 of GK2 (NP_149991).

  • Positive control
    • WB: H1299, HeLa or HepG2 cell lysate; ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab96818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance
    GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.
  • Cellular localization
    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.
  • Database links
  • Alternative names
    • ATP:glycerol 3 phosphotransferase antibody
    • EC 2.7.1.30 antibody
    • GK 2 antibody
    • GK antibody
    • GKP2 antibody
    • GKTA antibody
    • Glycerokinase antibody
    • Glycerol kinase 2 antibody
    • Glycerol kinase testis specific 2 antibody
    see all

Images

  • Anti-GK2 antibody (ab96818) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • 1: Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96818 at 1/200 dilution.
    2: image merged with DNA probe.

References

ab96818 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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