Overview

  • Product name
    Anti-GK2 antibody - C-terminal
    See all GK2 primary antibodies
  • Description
    Rabbit polyclonal to GK2 - C-terminal
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human GK2 conjugated to KLH.

  • Positive control
    • Human breast carcinoma tissue. Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.
  • Cellular localization
    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.
  • Database links
  • Alternative names
    • ATP:glycerol 3 phosphotransferase antibody
    • EC 2.7.1.30 antibody
    • GK 2 antibody
    • GK antibody
    • GKP2 antibody
    • GKTA antibody
    • Glycerokinase antibody
    • Glycerol kinase 2 antibody
    • Glycerol kinase testis specific 2 antibody
    see all

Images

  • Anti-GK2 antibody - C-terminal (ab71529) at 1/100 dilution + Jurkat cell lysate at 12.5 µg

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 39 kDa. We are unsure as to the identity of these extra bands.
  • ab71529 at 1/50 dilution, staining GK2 in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References

ab71529 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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