Overview

  • Product name
  • Description
    Rabbit polyclonal to Gli1
  • Specificity
    This antibody is specific for Gli1.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    A region within Synthetic peptide: TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP , corresponding to amino acids 1007-1056 of Human Gli1

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Gli1 peptide (ab111659) or Human Gli1 peptide (ab86150). 5% skim milk in PBS buffer. (An HRP conjugated anti-Rabbit IgG could be used as a secondary, a suggested dilution of 1/50000 to 1/100000). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • Function
    Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificity
    Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similarities
    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated in vitro by ULK3.
  • Cellular localization
    Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gli 1 antibody
    • GLI antibody
    • GLI family zinc finger 1 antibody
    • GLI Kruppel family member 1 antibody
    • gli1 antibody
    • GLI1_HUMAN antibody
    • Glioma associated oncogene 1 antibody
    • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
    • Glioma associated oncogene homolog antibody
    • Glioma-associated oncogene antibody
    • Oncogene GLI antibody
    • Zfp 5 antibody
    • Zfp5 antibody
    • Zinc finger protein GLI 1 antibody
    • Zinc finger protein GLI1 antibody
    see all

Anti-Gli1 antibody images

  • Lane 1 : MARKER
    Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 118 kDa
    Observed band size : 118 kDa
  • ICC/IF image of ab49314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa
    Observed band size : 118 kDa


    Exposure time : 20 minutes

    SKNSH cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of VU0155041 sodium salt (ab120248). Increased expression of Gli1 (ab49314) in SKNSH cells correlates with an decrease in VU0155041 sodium salt concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab49314 at 2.5 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti- rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.

  • Lane 1 : Anti-Gli1 antibody (ab49314) at 1.25 µg/ml
    Lane 2 : Anti-Gli1 antibody (ab49314) at 2.5 µg/ml
    Lane 3 : Anti-Gli1 antibody (ab49314) at 5 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 118 kDa

References for Anti-Gli1 antibody (ab49314)

This product has been referenced in:

See all 17 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Hydrogen Peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Uterus)
Specification
Uterus
Permeabilization
No
Fixative
Paraformaldehyde
Username

Hugo

Verified customer

Submitted Jul 18 2014

Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 09 2014

Abreviews
Application
Western blot
Loading amount
3 µg
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Sample
Rabbit Tissue lysate - whole (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (MKN45)
Specification
MKN45
Permeabilization
Yes - Triton 100X
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Specification
AGS
Sample
Human Cell lysate - whole cell (AGS)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Dayeon Yu

Verified customer

Submitted May 14 2013

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - .05% tween
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 19 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (limb sections)
Specification
limb sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization
Yes - 0.05% tween
Blocking step
5% goat serum 1% BSA in PBT as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C
Username

Mrs. Neta Maayan

Verified customer

Submitted Jul 18 2012

1-10 of 19 Abreviews or Q&A

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