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Anti-Gli2 antibody (ab7181)

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Overview

Product name

Anti-Gli2 antibody
See all Gli2 products (3) ...

Description

Rabbit polyclonal to Gli2

Specificity

ab7181 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected. We welcome feedback on this antibody.

Tested applications

IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide: RSKVKTEPEGLRPAS-C conjugated to KLH, corresponding to amino acids 46-60 of Human Gli2.

RSKVKTEPEG LRPAS-C

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Applications

Show applications key

Our Abpromise guarantee covers the use of ab7181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: 1/200. (PMID 19107131)
IHC-Fr: 1/100.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.

Involvement in disease

Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.

Post-translational
modifications

Phosphorylated in vitro by ULK3.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP10070 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Gli 2 antibody
  • GLI family zinc finger 2 antibody
  • GLI kruppel family member 2 antibody
  • GLI Kruppel family member GLI2 antibody
  • GLI2 antibody
  • GLI2_HUMAN antibody
  • Oncogene GLI2 antibody
  • Tax helper protein antibody
  • Tax helper protein 2 antibody
  • Tax responsive element 2 holding protein antibody
  • Tax responsive element 25 bp sequence binding protein antibody
  • THP 2 antibody
  • THP antibody
  • THP2 antibody
  • Zinc finger protein GLI2 antibody
see all

Database links

References for Anti-Gli2 antibody (ab7181)

This product has been referenced in:

  • Brunner Met al. Expression of hedgehog signaling molecules in Merkel cell carcinoma. Head Neck 32:333-40 (2010). IHC-P; Human.Read more (PubMed: 19644931) »
  • Ohta Het al. Cross talk between hedgehog and epithelial-mesenchymal transition pathways in gastric pit cells and in diffuse-type gastric cancers. Br J Cancer 100:389-98 (2009). IHC-P; Human.Read more (PubMed: 19107131) »

See all 2 publications for this product

Publishing research using ab7181? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"