Gli2 antibody (ab7181)

Product Name 

Gli2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Gli2

Immunogen 

Synthetic peptide: RSKVKTEPEGLRPAS-C conjugated to KLH, corresponding to amino acids 46-60 of Human Gli2.
BLAST 'RSKVKTEPEGLRPAS-C' with or

Reacts with 

Ms

Predicted to react 
(species key)

Hu
(Due to sequence homology)

Specificity 

ab7181 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4.

Tested by Western blotting on human lung, gives low background, but no band was detected. We welcome feedback on this antibody.

Tested applications 
(see key)

IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/100. Please see Abreview for method details.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

This protein belongs to the C2H2 type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes Greig cephalopolysyndactyly syndrome, Pallister Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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