Gli2 antibody (ab7195)

Product Name 

Gli2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Gli2

Immunogen 

Synthetic peptide: LRGAPSASRGSYTQQPR-C conjugated to KLH, corresponding to amino acids 1193-1209 of Mouse Gli2
BLAST 'LRGAPSASRGSYTQQPR-C' with or

Reacts with 
(species key)

Ms

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/15000 - 1/60000.
IHC-P: Use at a concentration of 2 - 20 µg/ml.
IHC-Fr: Use at an assay dependent dilution ~(PMID 17971852).
WB: 1/500 - 1/2000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

This protein belongs to the C2H2 type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes Greig cephalopolysyndactyly syndrome, Pallister Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Gli2 antibody images, references, products related to ab7195 and other tools.

Gli2 antibody images:

References for Gli2 antibody (ab7195)

  This product has been used in: (two most recent references)

Takanaga H et al. Gli2 Is A Novel Regulator of Sox2 Expression In Telencephalic Neuroepithelial Cells. Stem Cells : (2008). IHC-Fr; Mouse. PubMed: 18927476

Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr; Mouse. PubMed: 17971852

See all 3 publication references for this product.


If you publish research using ab7195 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Gli2 antibody

Customer FAQs regarding Gli2 antibody

Protocols for Gli2 antibody

Price and availability of products related to Gli2 antibody

Gli2 antibody - related products: