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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Gli2 antibody
See all Gli2 products (3) ...
Description
Rabbit polyclonal to Gli2
Tested applications
ELISA, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide: LRGAPSASRGSYTQQPR-C conjugated to KLH, corresponding to amino acids 1193-1209 of Mouse Gli2
LRGAPSASRG SYTQQPR-C
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Hedgehog >> Nuclear
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
IHC-Fr
Application notes
ELISA: 1/15000 - 1/60000.
IHC-P: Use at a concentration of 2 - 20 µg/ml.
IHC-Fr: Use at an assay dependent dilution ~(PMID 17971852).
WB: 1/500 - 1/2000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
Involvement in disease
Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Post-translational
modifications
Phosphorylated in vitro by ULK3.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP10070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gli 2 antibody
- GLI family zinc finger 2 antibody
- GLI kruppel family member 2 antibody
see all
Database links
- Entrez Gene: 14633 Mouse
- SwissProt: Q0VGT2 Mouse
- SwissProt: Q8K0K3 Mouse
- Unigene: 273292 Mouse
Anti-Gli2 antibody images:
Western blot - Gli2 antibody (ab7195)
All lanes : Anti-Gli2 antibody (ab7195)
Lane 1 : Mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate with Immunizing peptide
Lysates/proteins at 25 µg per lane.
Secondary
IRDye™800
conjugated Gt-a-Rabbit IgG (1/10000)
Observed band size : 190 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli2 antibody (ab7195)
ab7195 at 10 µg/ml staining mouse testis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Gli2 antibody (ab7195)
ab7195 at 10 µg/ml staining mouse brain.
Western blot - Anti-Gli2 antibody (ab7195)
developed using the ECL technique
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Gli2 protein levels were determined by WB in 2 adult mice in either cerebral cortex (CC) or in cerebellum (CB). Nuclear lysis buffer has to be used otherwise no bands can be detected. BSA blocking is better than milk.20µg lysate added per lane.ab7195 used at a 1/1000 dilution.Secondary used was an HRP conjugated goat anti-rabbit polyclonal used at a 1/10000 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-Gli2 antibody (ab7195)
This product has been referenced in:
- Zhang Zet al. Fuz Regulates Craniofacial Development through Tissue Specific Responses to Signaling Factors. PLoS One 6:e24608 (2011). IHC-P; Mouse.Read more (PubMed: 21935430) »
- Youssef KKet al. Identification of the cell lineage at the origin of basal cell carcinoma. Nat Cell Biol 12:299-305 (2010).Read more (PubMed: 20154679) »
See all 7 publications for this product
Publishing research using ab7195? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"