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Product name
Anti-Gli2 antibody - ChIP Grade
See all Gli2 products (3) ...
Description
Rabbit polyclonal to Gli2 - ChIP Grade
Specificity
This antibody recognises Gli2.
Tested applications
WB, IHC-P, ICC/IF, ICC, IHC-P, EMSA, IHC-Fr, ChIPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from a region between residues 600 - 649 of human Gli2.
.
RNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVED CLHVRAIKTE
Positive control
Jurkat cell lysate for Western blot, Human heart and human intestine tissue for Immunohistochemistry.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None.
Constituents: 2% Sucrose, PBS.
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 1.25 µg/ml. Predicted molecular weight: 133 kDa.Can be blocked with Gli2 peptide (ab130900). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P: Use a concentration of 4 - 8 µg/ml.
ICC/IF: Use at an assay dependent concentration.
ICC
ICC: 1/200. See AbreviewICC: 1/200. See Abreview
IHC-P: Use at an assay dependent dilution.
EMSA: Use a concentration of 1 µg/ml.
IHC-Fr: 1/100. See Abreview
ChIP: Use at an assay dependent dilution.
Target
Function
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
Involvement in disease
Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Post-translational
modifications
Phosphorylated in vitro by ULK3.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP10070
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gli 2 antibody
- GLI family zinc finger 2 antibody
- GLI kruppel family member 2 antibody
see all
Database links
- Entrez Gene: 2736 Human
- Entrez Gene: 14633 Mouse
- Omim: 165230 Human
- SwissProt: P10070 Human
- SwissProt: Q8K0K3 Mouse
- SwissProt: Q0VGT2 Mouse
- Unigene: 111867 Human
- Unigene: 273292 Mouse
see all
Anti-Gli2 antibody - ChIP Grade images:
Western blot - Gli2 antibody (ab26056)
Anti-Gli2 antibody - ChIP Grade (ab26056) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate 10ug.
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 133 kDa
Gel concentration: 10%
Immunohistochemistry (Paraffin-embedded sections) - Gli2 antibody (ab26056)
Ab26056 at 4-8 ug/ml staining paraffin embedded human heart tissue by Immunohistochemistry. Arrows indicate positively labelled myocardial cells. Magnification: x400.
Immunohistochemistry (Paraffin-embedded sections) - Gli2 antibody (ab26056)
Ab26056 at 4-8 ug/ml staining paraffin embedded human intestine tissue by Immunohistochemistry. Arrows indicate positively labelled epithelial cells of intestinal glands. Magnification: x400.
References for Anti-Gli2 antibody - ChIP Grade (ab26056)
This product has been referenced in:
- Yang L et al. Hedgehog signaling activation in the development of squamous cell carcinoma and adenocarcinoma of esophagus. Int J Biochem Mol Biol 3:46-57 (2012). IHC-FoFr; Rat.Read more (PubMed: 22509480) »
- Malek R et al. The p53 Inhibitor MDM2 Facilitates Sonic Hedgehog-Mediated Tumorigenesis and Influences Cerebellar Foliation. PLoS One 6:e17884 (2011). WB; Mouse.Read more (PubMed: 21437245) »
See all 18 publications for this product
Publishing research using ab26056? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"