Overview

  • Product nameAnti-Gliadin antibody [14D5]
    See all Gliadin primary antibodies
  • Description
    Mouse monoclonal [14D5] to Gliadin
  • SpecificityThis antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Common Wheat (Triticum aestivum), Barley (Hordeum vulgare)
  • Immunogen

    Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]

    KLQPFPQPELPYPQPQ

    ) coupled to immunogenic carrier protein.

  • EpitopeThe epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide.
  • General notes


    The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThe immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
  • ClonalityMonoclonal
  • Clone number14D5
  • Myelomax63-Ag8.653
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

dilution 1/1000

Target

  • RelevanceCeliac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods.
  • Cellular localizationCytoplasmic; major seed storage protein in wheat.
  • Alternative names
    • prolamin antibody

Anti-Gliadin antibody [14D5] images

  • All lanes : Anti-Gliadin antibody [14D5] (ab36729) at 1/1000 dilution

    Lane 1 : Rye Lysate
    Lane 2 : Barley Lysate
    Lane 3 : Wheat Lysate


    Performed under reducing conditions.

References for Anti-Gliadin antibody [14D5] (ab36729)

ab36729 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"