Overview

  • Product nameAnti-Gliomedin antibody
    See all Gliomedin primary antibodies
  • Description
    Rabbit polyclonal to Gliomedin
  • Specificityab24483 detects a Gliomedin sized band at 59kDa in WB.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat Gliomedin.

    (Peptide available as ab25746.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Brain (Mouse), Spinal Cord (Mouse), Brain (Rat) IF/ICC: PC12 cell line

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceGliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC.
  • Cellular localizationCell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • CANCER RELATED GENE LIVER 2 antibody
    • Cancer related gene-Liver 2 antibody
    • CLOM antibody
    • collomin antibody
    • Colm (gene name) antibody
    • COLM antibody
    • Colmedin antibody
    • CRG L2 antibody
    • CRGL2 antibody
    • FLJ23917 antibody
    • Gldn (gene name) antibody
    • UNC 112 antibody
    see all

Anti-Gliomedin antibody images

  • ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Gliomedin antibody (ab24483) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,30 kDa. We are unsure as to the identity of these extra bands.
    ab24483 detects a band of 70 kDa in Western Blot. Gliomedin contains a number of N-glycosylation sites (SwissProt data) which may explain the migration at a higher molecular weight than predicted based on primary sequence.

References for Anti-Gliomedin antibody (ab24483)

This product has been referenced in:
  • Kwon HS  et al. Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling. J Biol Chem 288:26357-71 (2013). Read more (PubMed: 23897819) »
  • Kawamura N  et al. Anti-neurofascin antibody in patients with combined central and peripheral demyelination. Neurology 81:714-22 (2013). Human . Read more (PubMed: 23884033) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab24483.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"