Overview

  • Product nameAnti-GLIPR2 antibody
    See all GLIPR2 primary antibodies
  • Description
    Rabbit polyclonal to GLIPR2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    antigen, corresponding to amino acids 2-73 (GKSASKQFHN EVLKAHNEYR QKHGVPPLKL CKNLNREAQQ YSEALASTRI LKHSPESSRG QCGENLAWAS YD) of Human GLIPR2 (Q9H4G4).

  • Positive control
    • Human lung tissue; Transfected HEK293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificityHighest expression in lung and peripheral leukocytes, and minor expression in liver and kidney.
  • Sequence similaritiesBelongs to the CRISP family.
    Contains 1 SCP domain.
  • Cellular localizationGolgi apparatus membrane. Binds lipid-enriched microdomains of Golgi membranes not only by ionic interactions but also through the myristate.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730414A08Rik antibody
    • C77180 antibody
    • C9orf19 antibody
    • GAPR 1 antibody
    • GAPR-1 antibody
    • GAPR1_HUMAN antibody
    • GLI pathogenesis related 2 antibody
    • Glioma pathogenesis related protein 2 antibody
    • Glioma pathogenesis-related protein 2 antibody
    • GliPR 2 antibody
    • GLIPR2 antibody
    • Golgi associated plant pathogenesis related protein 1 antibody
    • Golgi associated PR 1 protein antibody
    • Golgi-associated plant pathogenesis-related protein 1 antibody
    • Golgi-associated PR-1 protein antibody
    • OTTMUSP00000007558 antibody
    • RP11-421H8.5 antibody
    • RP23-209M8.2 antibody
    see all

Anti-GLIPR2 antibody images

  • All lanes : Anti-GLIPR2 antibody (ab122059) at 1/1000 dilution

    Lane 1 : Mouse cell lysate
    Lane 2 : Human cell lysate
    Lane 3 : Human cell lysate

    Lysates/proteins at 20000000 cells per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-GLIPR2 antibody (ab122059) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : GLIPR2-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique
  • ab121326, at 1/15, staining GLIPR2 in paraffin embedded Human lung tissue by Immunohistochemistry.

References for Anti-GLIPR2 antibody (ab122059)

ab122059 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 2e+007 cells
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - other (N/A)
Specification N/A
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"