Overview

  • Product nameAnti-GliS2 antibody
    See all GliS2 primary antibodies
  • Description
    Rabbit polyclonal to GliS2
  • Specificityab28462 recognises GLIS2.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide derived from residues 183 - 232 of human GliS2.

    .

  • Positive control
    • HepG2 cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • FunctionCan act either as a transcription repressor or as a transcription activator, depending on the cell context. Represses the transcriptional activation mediated by CTNNB1 in the Wnt pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.
  • Tissue specificityExpressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon.
  • Involvement in diseaseDefects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7) [MIM:611498]. NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.
  • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • DomainThe C2H2-type zinc finger 1 has a major repressor function and is required for CTNNB1 binding.
  • Post-translational
    modifications
    C-terminus cleavage is induced by interaction with CTNND1 and enhanced by Src tyrosine kinase.
  • Cellular localizationNucleus speckle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ38247 antibody
    • GLI similar 2 antibody
    • GLI-similar 2 antibody
    • GLIS 2 antibody
    • GLIS family zinc finger 2 antibody
    • glis2 antibody
    • GLIS2_HUMAN antibody
    • Kruppel like zinc finger protein GLIS2 antibody
    • Neuronal Krueppel-like protein antibody
    • Zinc finger protein GLIS2 antibody
    see all

Anti-GliS2 antibody images

  • Anti-GliS2 antibody (ab28462) at 0.5 µg/ml

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

References for Anti-GliS2 antibody (ab28462)

ab28462 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The difference between ab48861 and ab28462 can be found in the purity and concentration. I have listed the differences below and some references that might be useful to you. ab48861 purity: protein A purified concent...

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