Anti-GLIS3 antibody (ab51268)

Overview

  • Product nameAnti-GLIS3 antibody
    See all GLIS3 primary antibodies
  • Description
    Rabbit polyclonal to GLIS3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    A region within synthetic peptide: MMVQRLGLIS PPASQVSTAC NQISPSLQRA MNAANLNIPP SDTRSLISRE S, corresponding to N terminal amino acids 1-50 of Human GLIS3

  • Positive control
    • HepG2 cell lyaste.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab51268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceGLIS3 is a member of the GLI similar zinc finger protein family, and encodes a nuclear protein with five C2H2 type zinc finger domains. It functions as both an activator and repressor of transcription, and is specifically involved in the development of pancreatic beta cells, thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full length nature of only two have been determined.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • FLJ38999 antibody
    • FLJ90578 antibody
    • GLI similar 3 antibody
    • GLI SIMILAR PROTEIN 3 antibody
    • GLIS family zinc finger 3 antibody
    • MGC33662 antibody
    • OK/KNS-cl.4 antibody
    • Zinc finger protein 515 antibody
    • Zinc finger protein GLIS3 antibody
    • ZNF515 antibody
    see all

References for Anti-GLIS3 antibody (ab51268)

ab51268 has not yet been referenced specifically in any publications.

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Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab51268. I would also appreciate if you can confirm some further details:
1. Have the cells you have been using been frequently passaged? If s...

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Thank you very much for your interest in ab51268.

We do not currently have an image of ab51268 in WB. Therefore, I can offer a discount off a future purchase if you buy ab51268 now, use it WB and submit an image of your results to us in the f...

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Application Western blot
Sample Rat Tissue lysate - whole (PANCREAS)
Loading amount 30 µg
Specification PANCREAS
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"