Recombinant full length Mouse GLO1 protein with a tag.
ab81461 also reacts with Simian.
Our Abpromise guarantee covers the use of ab81461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20627935|
|WB||1/1000. Predicted molecular weight: 21 kDa.|
|Flow Cyt||Use 1µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab81461 staining human ovarian carcinoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab81461 at 1/500 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rat polyclonal antibody at 1/200 was used as the secondary antibody.