Overview

  • Product nameAnti-GLO1 antibody [6F10]
    See all GLO1 primary antibodies
  • Description
    Rat monoclonal [6F10] to GLO1
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-Fr, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Mouse GLO1 protein with a tag.

  • Positive control
    • WB: COS-1, L929 and HeLa whole cell extracts. ICC/IF: Hela cells.
  • General notes


    ab81461 also reacts with Simian.

Properties

Applications

Our Abpromise guarantee covers the use of ab81461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20627935
WB 1/1000. Predicted molecular weight: 21 kDa.
Flow Cyt Use 1µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
  • PathwaySecondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
  • Sequence similaritiesBelongs to the glyoxalase I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aldoketomutase antibody
    • glo1 antibody
    • GLOD1 antibody
    • Glx I antibody
    • GLYI antibody
    • glyoxalase domain containing 1 antibody
    • Glyoxalase I antibody
    • Ketone aldehyde mutase antibody
    • Ketone-aldehyde mutase antibody
    • Lactoyl glutathione lyase antibody
    • Lactoylglutathione lyase antibody
    • LGUL_HUMAN antibody
    • Methylglyoxalase antibody
    • S D lactoylglutathione methylglyoxal lyase antibody
    • S-D-lactoylglutathione methylglyoxal lyase antibody
    see all

Anti-GLO1 antibody [6F10] images

  • Immunofluorescent staining of HeLa cells with ab81461.
  • All lanes : Anti-GLO1 antibody [6F10] (ab81461)

    Lane 1 : COS-1 (simian) whole cell extract
    Lane 2 : L929 (mouse) whole cell extract
    Lane 3 : HeLa (human) whole cell extract


    Predicted band size : 21 kDa
    Observed band size : 27-29 kDa (why is the actual band size different from the predicted?)
  • ab81461 staining human ovarian carcinoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab81461 at 1/500 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rat polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab81461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-GLO1 antibody [6F10] (ab81461)

This product has been referenced in:
  • Chugh G  et al. A high-salt diet further impairs age-associated declines in cognitive, behavioral, and cardiovascular functions in male Fischer brown Norway rats. J Nutr 143:1406-13 (2013). Read more (PubMed: 23864508) »
  • Bento CF  et al. Methylglyoxal-induced imbalance in the ratio of vascular endothelial growth factor to angiopoietin 2 secreted by retinal pigment epithelial cells leads to endothelial dysfunction. Exp Physiol 95:955-70 (2010). WB ; Human . Read more (PubMed: 20562294) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Rat Tissue sections (DRG)
Specification DRG
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (DRG)
Specification DRG
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 20 2013

Thank you for contacting us. I am sorry that ab81461 is not producing satisfactory results on mouse brain sections. We have several other antibodies to GLO1, but none of the others have been validated for use on frozen tissue sections, and only the rab...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ovarian Carcinoma)
Specification Ovarian Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"