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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
1/100 - 1/250.
1/100 - 1/200.
Function Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
Pathway Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities Belongs to the glyoxalase I family.
Information by UniProt
Anti-GLO1 antibody images
Western blot - GLO1 antibody (ab96032)
All lanes : Anti-GLO1 antibody (ab96032) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : H1299 whole cell lysate Lane 4 : HeLa whole cell lysate Lane 5 : HepG2 whole cell lysate Lane 6 : MOLT4 whole cell lysate Lane 7 : Raji whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GLO1 antibody (ab96032)
Immunohistochemical analysis of GLO1 in paraffin embedded ovarian carcinoma tissue, using ab96032 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - GLO1 antibody (ab96032)
Immunofluorescence analysis of GLO1 in paraformaldehyde fixed HeLa, using ab96032 at a 1/200 dilution. Lower image: merged with DNA probe.
References for Anti-GLO1 antibody (ab96032)
This product has been referenced in:
et al. Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates. Sci Rep 6:37737 (2016).
Read more (PubMed: 27898103) »
See 1 Publication
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"