Global Protein Sumoylation Assay Kit (ab115131)

Overview

  • Product nameGlobal Protein Sumoylation Assay Kit
    See all Sumoylation kits
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: all Mammals
  • Product overview

    Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners. Thus, detection of in vivo protein sumoylation (SUMO conjugation) would provide useful information for understanding SUMO modification as a regulatory control mechanisms for many nuclear proteins.


    Global Protein Sumoylation Assay Kit (ab115131) allows the user to measure in vivo protein sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo protein sumoylation from multiple mammalian cells/tissues extracts.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab115131 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Global Protein Sumoylation Assay Kit (ab115131)

    ab115131 has not yet been referenced specifically in any publications.

    Product Wall

    Total cell extracts can be used. However using nuclear proteins is better than using total cell extracts as the sumoylation is mainly with nuclear proteins such as HDACs. The extraction protocol of EpiSeeker Nuclear Extraction Kit (http://www.abcam.com...

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    The antibody used in the kit is mouse mAb.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    You may find the information you need about the EpiSeeker Global Protein Sumoylation Assay Kitby looking at the kit datasheet (http://www.abcam.com/ab115131) or at the kit protocol booklet available via this link: h...

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    You will want control wells for each of your treated samples. Therefore if you have ten different treatments plus one 'Untreated' control you will also want eleven 'No Antibody Controls' (ten from different tre...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"