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Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners. Thus, detection of in vivo protein sumoylation (SUMO conjugation) would provide useful information for understanding SUMO modification as a regulatory control mechanisms for many nuclear proteins.
Global Protein Sumoylation Assay Kit (ab115131) allows the user to measure in vivo protein sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo protein sumoylation from multiple mammalian cells/tissues extracts.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 25ml||2 x 25ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|8-Well Control Strips||2 units||3 units|
|Binding Buffer||1 x 15ml||1 x 30ml|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Color Development Solution||1 x 6ml||1 x 12ml|
|Negative Control, 1 µg/µL||1 x 120µl||1 x 240µl|
|Signal Report Solution||1 x 5µl||1 x 10µl|
|Stop Solution||1 x 3ml||1 x 6ml|
|Sumo Antibody, 0.5 µg/µL||1 x 20µl||1 x 40µl|
|Sumo Assay Buffer||1 x 2ml||1 x 4ml|
|Sumo Protein, 1 µg/µL||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115131 has not yet been referenced specifically in any publications.