Overview

  • Product name
  • Description
    Rabbit polyclonal to GLS2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human GLS2 aa 550 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9UI32

  • Positive control
    • This antibody gave a positive signal in Mouse Liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab150474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).

Target

  • Function
    Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
  • Tissue specificity
    Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
  • Sequence similarities
    Belongs to the glutaminase family.
    Contains 2 ANK repeats.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • breast cell glutaminase antibody
    • GA antibody
    • GLS antibody
    • Gls2 antibody
    • GLSL_HUMAN antibody
    • glutaminase 2 (liver mitochondrial) antibody
    • glutaminase 2 antibody
    • glutaminase GA antibody
    • glutaminase I antibody
    • Glutaminase liver isoform antibody
    • Glutaminase liver isoform, mitochondrial antibody
    • hLGA antibody
    • L glutaminase antibody
    • L glutamine amidohydrolase antibody
    • L-glutaminase antibody
    • L-glutamine amidohydrolase antibody
    • LGA antibody
    • mitochondrial antibody
    • phosphate activated glutaminase antibody
    • phosphate-dependent glutaminase antibody
    see all

Images

  • Anti-GLS2 antibody (ab150474) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 66 kDa


    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab150474 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab150474 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Apr 07 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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