Overview

  • Product name
  • Description
    Rabbit polyclonal to GLT25D2
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to amino acids 271-344 (AFSSRQAGIQ MYLCNREHYG YLPIPLKPHQ TLQEDIENLI HVQIEAMIDR PPMEPSQYVS VVPKYPDKMG FDEI) of Human GLT25D2.

  • Positive control
    • Human breast tissue; RT-4, U-251 MG, Human liver and tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues on collagen.
  • Tissue specificity
    Expressed in brain and skeletal muscle.
  • Sequence similarities
    Belongs to the glycosyltransferase 25 family.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1orf17 antibody
    • COLGALT2 antibody
    • Collagen beta(1 O)galactosyltransferase 2 antibody
    • Glt25d2 antibody
    • Glycosyltransferase 25 domain containing 2 antibody
    • Glycosyltransferase 25 family member 2 antibody
    • GT252_HUMAN antibody
    • Hydroxylysine galactosyltransferase 2 antibody
    • Procollagen galactosyltransferase 2 antibody
    • RP11-498P10.2 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and endoplasmic reticulum. Recommended concentration of ab122192 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122192 at 1/350 dilution staining GLT25D2 in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
  • All lanes : Anti-GLT25D2 antibody (ab122192) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 51 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab122192 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mammary glands)
Specification
Mammary glands
Username

Abcam user community

Verified customer

Submitted Feb 17 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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