Overview

  • Product nameAnti-Glucagon antibody (Biotin)
    See all Glucagon primary antibodies
  • Description
    Rabbit polyclonal to Glucagon (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IP, ELISA, RIA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    HSQGTFTSDYSKYLDSRRAQDFVQWLMNT

    conjugated to KLH via carboxyl group, corresponding to internal sequence amino acids 53-81 of Human Glucagon.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab48287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Glucagon antibody (Biotin) images

  • IHC image of Glucagon staining in THuman normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48287, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Glucagon antibody (Biotin) (ab48287)

ab48287 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab48287.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"