Overview

  • Product nameAnti-Glucagon antibody [EP3070]
    See all Glucagon primary antibodies
  • Description
    Rabbit monoclonal [EP3070] to Glucagon
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control
    • HepG2, Human fetal liver, Mouse kidney and Mouse spleen lysates. Human pancreas tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Glucagon antibody (BSA and Azide free) [EP3070] - (ab167078)

Properties

Applications

Our Abpromise guarantee covers the use of ab92517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • RelevanceGlucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • GCG antibody

    Anti-Glucagon antibody [EP3070] images

    • Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.

      See Abreview

    • ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
    • All lanes : Anti-Glucagon antibody [EP3070] (ab92517)

      Lane 1 : HepG2 Lysate
      Lane 2 : Human Fetal liver lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen Lysate


      Predicted band size : 21 kDa

    References for Anti-Glucagon antibody [EP3070] (ab92517)

    This product has been referenced in:
    • Ortmann J  et al. Immunohistochemical methods as an aid in estimating the time since death. Forensic Sci Int 273:71-79 (2017). IHC-P ; Human . Read more (PubMed: 28236731) »
    • Du W  et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27751232) »

    See all 6 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Pancreas)
    Permeabilization No
    Specification Pancreas
    Blocking step serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted Mar 24 2017

    Application ELISA
    Sample Human Recombinant protein (Synthetic peptide)
    Specification Synthetic peptide
    Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type Indirect
    Username

    Abcam user community

    Verified customer

    Submitted Mar 01 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (pancreas)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH 6.0
    Permeabilization No
    Specification pancreas
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"