• Product nameAnti-Glucagon antibody [EP3070]
    See all Glucagon primary antibodies
  • Description
    Rabbit monoclonal [EP3070] to Glucagon
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control
    • HepG2, Human fetal liver, Mouse kidney and Mouse spleen lysates. Human pancreas tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Glucagon antibody (BSA and Azide free) [EP3070] - (ab167078)



Our Abpromise guarantee covers the use of ab92517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • RelevanceGlucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • GCG antibody

    Anti-Glucagon antibody [EP3070] images

    • Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.

      See Abreview

    • ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
    • All lanes : Anti-Glucagon antibody [EP3070] (ab92517)

      Lane 1 : HepG2 Lysate
      Lane 2 : Human Fetal liver lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen Lysate

      Predicted band size : 21 kDa

    References for Anti-Glucagon antibody [EP3070] (ab92517)

    This product has been referenced in:
    • Ortmann J  et al. Immunohistochemical methods as an aid in estimating the time since death. Forensic Sci Int 273:71-79 (2017). IHC-P ; Human . Read more (PubMed: 28236731) »
    • Du W  et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27751232) »

    See all 6 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Pancreas)
    Permeabilization No
    Specification Pancreas
    Blocking step serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 24 2017

    Application ELISA
    Sample Human Recombinant protein (Synthetic peptide)
    Specification Synthetic peptide
    Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type Indirect

    Abcam user community

    Verified customer

    Submitted Mar 01 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (pancreas)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH 6.0
    Permeabilization No
    Specification pancreas
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Sep 12 2015