Recombinant Anti-Glucagon antibody [EPR3070-45] (ab108426)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3070-45] to Glucagon
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucagon antibody [EPR3070-45]
See all Glucagon primary antibodies -
Description
Rabbit monoclonal [EPR3070-45] to Glucagon -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Glucagon recombinant protein; Human pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.31 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3070-45 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Predicted molecular weight: 21 kDa.
For unpurified, use 1/2000. |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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WB
1/5000. Predicted molecular weight: 21 kDa. For unpurified, use 1/2000. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2641 Human
- Omim: 138030 Human
- SwissProt: P01275 Human
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Alternative names
- GCG antibody
Images
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Immunohistochemical staining of paraffin embedded human pancreas with purified ab108426 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Unpurified ab108426, at 1/100 dilution, staining Glucagon in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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Anti-Glucagon antibody [EPR3070-45] (ab108426) at 1/2000 dilution (unpurified) + Glucagon recombinant protein at 10 µg
Predicted band size: 21 kDa -
Unpurified ab108426 showing negative staining in Skeletal muscle tissue.
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Unpurified ab108426 showing negative staining in Normal lung tissue.
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Unpurified ab108426 showing negative staining in Normal kidney tissue.
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Unpurified ab108426 showing negative staining in Normal brain tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108426 has not yet been referenced specifically in any publications.