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Polymerized porcine glucagon.
Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340. Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Our Abpromise guarantee covers the use of ab10988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21821034|
|RIA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
Immunohistochemical analysis of Mouse pancreas tissue sections labeling Glucagon with ab10988 at 1/1000 dilution
Immunohistochemical analysis of Mouse pancreas tissue labeling Glucagon with ab10988 at 1/1000 dilution.
ab10988 staining human islets (beta cells) by ICC/IF. Cells were PFA fixed and blocked in 2% serum for 1 hour at 25°C prior to incubating with ab10988 for 1 hour at 25°C. A Cy3® conjugated goat anti-mouse antibody diluted 1/500 was used as the secondary.