Overview

  • Product name
    Anti-Glucagon antibody [K79bB10]
    See all Glucagon primary antibodies
  • Description
    Mouse monoclonal [K79bB10] to Glucagon
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Fr, RIA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig, Common marmoset
    Predicted to work with: Rabbit, Guinea pig, Cat, Syrian hamster
  • Immunogen

    Polymerized porcine glucagon.

  • Positive control
    • Pancreatic glucagon for RIA.
  • General notes

    Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340.  Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21821034
RIA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

Target

Images

  • ab10988 at 1/200 staining mouse pancreas tissue sections by IHC-P. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours at 4°C. A Texas Red conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of Mouse pancreas tissue sections labeling Glucagon with ab10988 at 1/1000 dilution

    See Abreview

  • Immunohistochemical analysis of Mouse pancreas tissue labeling Glucagon with ab10988 at 1/1000 dilution.

    See Abreview

  • ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.

    See Abreview

  • ab10988 staining human islets (beta cells) by ICC/IF.  Cells were PFA fixed and blocked in 2% serum for 1 hour at 25°C prior to incubating with ab10988 for 1 hour at 25°C.  A Cy3® conjugated goat anti-mouse antibody diluted 1/500 was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Monkey . Read more (PubMed: 28190929) »
  • Roth Flach RJ  et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30 (2016). IHC-P ; Mouse . Read more (PubMed: 27226575) »

See all 39 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

For ab10988: ICC/IF was added based on published references and a customer Abreview. Thus, a protocol would be based on these sources. We have not tested it ourselves in this application.

The abreview i...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Formalin
Username

Miss. Barbara Makela

Verified customer

Submitted Jun 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (pancreas)
Antigen retrieval step
None
Permeabilization
No
Specification
pancreas
Blocking step
dako antibody diluent with background reducing components as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 03 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syrian Hamster Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 28 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 28 2015

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you in regards to the Anti-Glucagon antibody [K79bB10] (ab10988).

Glucagon is cleaved into 8 peptide chains one of them is GLP1. As this...

Read More

Thank you for contacting us.

I have contacted the lab regarding the 4 antibodies you had inquired about, and will be in touch with more informaton as soon as I have it.

Thank you for your patience!


Use our products...

Read More

Thank you for contacting us. I was able to find a paper describing production and characterization of the ab10988 clone, K79bB10, but as far as I can tell, the epitope has not been mapped. Here is a link to the paper's abstract: http://www.ncbi.n...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Dr. Miri Stolovich-Rain

Verified customer

Submitted Nov 09 2011

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up