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Our Abpromise guarantee covers the use of ab189279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 21 kDa.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas, islets of Langerhans labeling Glucagon with ab189279 at 10 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue labeling Glucagon with ab189279 at 10 µg/ml, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells labeling Glucagon with ab189279 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab189279 has not yet been referenced specifically in any publications.