Overview

  • Product name
    Anti-Glucagon Receptor antibody
    See all Glucagon Receptor primary antibodies
  • Description
    Rabbit polyclonal to Glucagon Receptor
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Glucagon Receptor.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Caco2; HepG2; SW480.

Properties

Applications

Our Abpromise guarantee covers the use of ab109279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Function
    This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gcgr antibody
    • GGR antibody
    • GL R antibody
    • GL-R antibody
    • GLR antibody
    • GLR_HUMAN antibody
    • Glucagon receptor antibody
    • Glucagon receptor precursor antibody
    • MGC138246 antibody
    see all

Images

  • All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 25 kDa,51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab109279 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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