Anti-Glucocorticoid Receptor antibody [3D5] (ab9568)

Overview

  • Product nameAnti-Glucocorticoid Receptor antibody [3D5]
    See all Glucocorticoid Receptor primary antibodies
  • Description
    Mouse monoclonal [3D5] to Glucocorticoid Receptor
  • SpecificityThis antibody recognizes Glucocorticoid Receptor It does not react with TGB, BSA and KLH.
  • Tested applicationsSuitable for: ELISA, Flow Cyt, ICC, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide fragment of a well conserved region of the human corticosteroid receptor (amino acid sequence 150-175, Mw: 2719,

    APTEKEFPKTHSDVSSEQQHLKGQTG

    ).

  • EpitopeGCR fragment 150-175, as well as, GCR isolated from HEP/G2 cells cultured in vitro using both simple binding and competition ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab9568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. A permeabilization step is recommended to detect GCR receptors in peripheral blood and bone marrow cells
Flow Cyt 1/100 - 1/1000. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 86 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody [3D5] images

  • Overlay histogram showing Jurkat cells stained with ab9568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9568, 1/1000) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical detection of GCR producing cells in formalin-fixed, paraffin embedded human breast carcinoma tissue sections with ab9568.
  • ab9568 staining human primary neuron cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X 100 in PBST (0.1%Tween) for 30 mins at RT prior to blocking with 10% normal donkey serum for 2 hrs at 21°C.  The primary antibody was used at a concentration of 5 µg/ml and incubated with the sample for 18 hrs at 4°C.  An Alexa Fluor® 546 conjugated donkey anti-mouse antibody was used as the secondary.

    Cells were counterstained with MAP2 and Neurofilament -- Green, and Hoecht's - Blue.  Image is 40X, 3D deconvolved 10 planes at 5um steps.

    See Abreview

References for Anti-Glucocorticoid Receptor antibody [3D5] (ab9568)

This product has been referenced in:
  • Uchida T  et al. Selective loss of parvalbumin-positive GABAergic interneurons in the cerebral cortex of maternally stressed Gad1-heterozygous mouse offspring. Transl Psychiatry 4:e371 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24618690) »
  • Lambert WM  et al. Brain-derived neurotrophic factor signaling rewrites the glucocorticoid transcriptome via glucocorticoid receptor phosphorylation. Mol Cell Biol 33:3700-14 (2013). Read more (PubMed: 23878391) »

See all 9 Publications for this product

Product Wall

Thank you for your inquiry.

We have currently three secondary antibodies that match your specifications:

ab97239

http://www.abcam.com/index.html?datasheet=97239 (or use the following: http://www.abcam.com/index.html?datash...

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These cell lines are uninduced and whole cell lysates.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I do not yet have the western blot data for this GR antibody from the lab that validated it, but I can recommend lysates of HeLa and several other cell lines, based on the western blot for a different GR antibody...

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I have not heard back from the laboratory that validated this antibody for western blotting. We do list at least two references on the datasheet in which it was used for western blotting, the references that are ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative paraffin
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Jan 18 2012

My colleague Tom told me that you called today and discussed the lab's staining protocol, so I want to follow-up to see if the antigen retrieval alteration improves the results or not. Please keep me updated about the results, and if the staining is st...

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Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - ra...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Primary Neuron)
Specification Primary Neuron
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X 100 in PBST (0.1%Tween) 30 minutes, rt
Blocking step Normal Donkey Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Dr. Erick Tatro

Verified customer

Submitted Mar 05 2008

Thank you for taking the time to fill out our technical questionaire and send it in to us. I am sorry you have had difficulties obtaining satisfactory results from this antibody (ab9568). I have searched further into the details of Glucocorticoid r...

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