Anti-Glucocorticoid Receptor antibody (ab55400)

Overview

  • Product nameAnti-Glucocorticoid Receptor antibody
    See all Glucocorticoid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Glucocorticoid Receptor
  • Specificityab55400 detects endogenous levels of total Glucocorticoid Receptor protein.
  • Tested applicationsSuitable for: WB, ELISA, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Glucocorticoid Receptor around the phosphorylation site of serine 226 (L-L-S-P-L).

  • Positive control
    • Jurkat cell extract, treated with EGF (200ng/ml, 15mins). IF/ICC: PC12 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab55400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ELISA 1/10000.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody images

  • All lanes : Anti-Glucocorticoid Receptor antibody (ab55400) at 1/500 dilution

    Lane 1 : Jurkat cell extract, treated with EGF (200ng/ml, 15mins), without immunizing peptide
    Lane 2 : Jurkat cell extract, treated with EGF (200ng/ml, 15mins), with immunizing peptide


    Predicted band size : 86 kDa
    Observed band size : 86 kDa
  • ab55400 immunoprecipitating Glucocorticoid Receptor from Hepa1-6 (murine hepatoma) cells. 1 mg of cell lysate was incubated with the primary antibody and matrix (Protein A) at a concentration of 20 µl/mg lysate for 18 hours at 4°C in cell lysis buffer. Immunoprecipitate was then Western blotted with ab55400 at 1/500.

    See Abreview

  • ICC/IF image of ab55400 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55400, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Glucocorticoid Receptor antibody (ab55400)

ab55400 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 20 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Total protein in input 1000 µg
Specification Hepa1-6 (murine hepatoma) cells
Immuno-precipitation step Protein A
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Submitted May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Loading amount 50 µg
Specification Hepa1-6 (murine hepatoma) cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

Submitted Apr 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"