Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3578)

Overview

  • Product nameAnti-Glucocorticoid Receptor antibody - ChIP Grade
    See all Glucocorticoid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Glucocorticoid Receptor - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Inhibition Assay, ChIP, Gel supershift assays, IP, ICC, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Human, Saccharomyces cerevisiae, Non Human Primates
    Predicted to work with: Guinea pig, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 346-367.
    Sequence:

    DQKPIFNVIPPIPVGSENWNRC


    (Peptide available as ab5019)

Properties

Applications

Our Abpromise guarantee covers the use of ab3578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20.
IHC-P 1/200.
Inhibition Assay Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
Gel supershift assays Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.

Immunocytochemical staining of GR in HeLa cells results in cytoplasmic staining in the absence of ligand, and nuclear staining after hormone administration.

WB Use a concentration of 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 86 kDa).Can be blocked with Glucocorticoid Receptor peptide (ab5019).
IHC-FoFr Use at an assay dependent concentration. PubMed: 18279320

Target

  • FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody - ChIP Grade images

  • Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of NIH-3T3 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • ab3578 staining Glucocorticoids Receptors in Mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in 4% paraformaldehyde and 15µm sections were cut using cryostat. The primary antibody was diluted, 1/100 in TNB buffer and incubated with sample for 18 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary. Red staining repersents staining with ab3578 in perilesional cells. 

    See Abreview

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3578)

This product has been referenced in:
  • Zinkhan EK  et al. Maternal tobacco smoke increased visceral adiposity and serum corticosterone levels in adult male rat offspring. Pediatr Res 76:17-23 (2014). WB ; Rat . Read more (PubMed: 24727947) »
  • Du J  et al. Ginsenoside Rg1, a novel glucocorticoid receptor agonist of plant origin, maintains glucocorticoid efficacy with reduced side effects. J Immunol 187:942-50 (2011). WB ; Human . Read more (PubMed: 21666059) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry and interest in our products.
The immunogen sequence used to raise this antibody (ab3578: Anti-Glucocorticoid Receptor) was DQKPIFNVIPPIPVGSENWNR, corresponding to amino acids 346-367 of Human Glucocorticoid Receptor. Ho...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Apr 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (Expression of Human Glucocorticoid Receptor in CHO)
Specification Expression of Human Glucocorticoid Receptor in CHO
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2008

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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