Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)

Overview

  • Product nameAnti-Glucocorticoid Receptor antibody - ChIP Grade
    See all Glucocorticoid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Glucocorticoid Receptor - ChIP Grade
  • Tested applicationsSuitable for: ChIP, Gel supershift assays, Flow Cyt, ICC, IP, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Non Human Primates
    Predicted to work with: Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 245-259.
    Sequence:

    CKPLILPDTKPKIKD


    (Peptide available as ab5833)

Properties

Applications

Our Abpromise guarantee covers the use of ab3579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. See Lerner et al., 2003.
Gel supershift assays Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Glucocorticoid Receptor peptide (ab5833). Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate.
IHC-Fr Use a concentration of 1 µg/ml. Staining of GR in rat hippocampus results in staining primarily in the nucleus.
ICC/IF 1/40. PubMed: 19202080

Target

  • FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody - ChIP Grade images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

References for Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)

This product has been referenced in:
  • Wang B  et al. Chondrocyte ß-catenin signaling regulates postnatal bone remodeling through modulation of osteoclast formation in a murine model. Arthritis Rheumatol 66:107-20 (2014). ChIP . Read more (PubMed: 24431282) »
  • Jones CL  et al. Loss of TBL1XR1 Disrupts Glucocorticoid Receptor Recruitment to Chromatin and Results in Glucocorticoid Resistance in a B-Lymphoblastic Leukemia Model. J Biol Chem 289:20502-20515 (2014). WB ; Human . Read more (PubMed: 24895125) »

See all 13 Publications for this product

Product Wall

Thank you very much for this extra information, this is very useful indeed for our investigations.

Please do not hesitate to inform me if the replacement antibody gave you satisfactory results or not.

Thank you foryour cooperation.

I am sorry ab3578 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of ab3579. The order number ****. <...

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Thank you for your enquiry and your interest in our products.
This antibody has not been characterized for ChIP in our lab. The ChIP application has been added to the datasheet based on this specific article:
http://www.ncbi.nlm.nih.gov/pubme...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (LNCaP-1F5 Cell line)
Specification LNCaP-1F5 Cell line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control PSA enhancer ~4 kb upstream of TSS
Negative control Negative control region on Chromosome X not known to be bound by any nuclear receptors.
Username

Abcam user community

Verified customer

Submitted Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP-1F5 Cell line and A549)
Loading amount 40 µg
Specification LNCaP-1F5 Cell line and A549
Treatment Ethanol, 100 nM Dexamethasone, in LNCaP-1F5 and A549 - 24 hours
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: +4°C
Username

Dr. Biswajyoti Sahu

Verified customer

Submitted Nov 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Neurons)
Loading amount 20 µg
Specification Neurons
Gel Running Conditions Reduced Denaturing (8% PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (skin epidermis)
Total protein in input 500 µg
Specification skin epidermis
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

Submitted Oct 06 2008

Thank you for your interests in our products. Yes, we have a product (ab3580) that specifically detects GR-alpha from human, mouse and rat tissues and does not detect GR-beta isoform. I have attached a datasheet link to this product for your easy a...

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Thank you for your patience. I have received a reply from the originator of ab3579 ((Glucocorticoid Receptor Antibody)). At this time, we do not have any data regarding the affinity constant of this antibody. I apologize for any inconvenience th...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit polyclonal to Glucocorticoid Receptor (ab3579) is a good selling antibody. It is yet to be tested by IHC using paraffin embedded sectio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"