Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)

Overview

  • Product nameAnti-Glucocorticoid Receptor antibody - ChIP Grade
    See all Glucocorticoid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Glucocorticoid Receptor - ChIP Grade
  • SpecificityThis antibody detects both the unactivated and activated forms of GR.
  • Tested applicationsSuitable for: IHC-P, ChIP, ICC/IF, Gel supershift assays, ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Non Human Primates, Reptiles
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 150-175.
    Sequence:

    APTEKEFPKTHSDVSSEQQHLKGQTG

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
ChIP Use at an assay dependent concentration.
ICC/IF 1/100.
Gel supershift assays Use at an assay dependent concentration.
ICC 1/100.
IP Use at an assay dependent concentration.
WB 1/100. Detects a band of approximately 97 kDa (predicted molecular weight: 86 kDa).

Target

  • FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor antibody - ChIP Grade images

  • Immunocytochemistry/Immunofluorescence analysis of 293 cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.

  • All lanes : Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671) at 1/1000 dilution

    Lane 1 : Cell lysate prepared from rabbit reticulocyte transfected with human GR recombinant protein.
    Lane 2 : Cell lysate prepared from mock translated lysate.

    Lysates/proteins at 2 µg per lane.

    Secondary
    HRP conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 86 kDa
    Observed band size : 94 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

References for Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)

ab3671 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HELA)
Loading amount 50 µg
Specification HELA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Verified customer

Submitted Jan 16 2012

Thank you for submitting you abreview for ab3671. I'm sorry that it has not performed as expected with the Western blotting or the ICC. Having reviewed the protocol there may be a few things I can suggest in order to improve the results. As you...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HELA)
Specification HELA
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TRITON-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (in vitro translated protein using rabbit reticuloc)
Loading amount 2 µg
Specification in vitro translated protein using rabbit reticuloc
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 500 µg
Specification HeLa cells
Treatment 1uM dex for 12 hours
Immuno-precipitation step Protein A/G
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Submitted Feb 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"