Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)
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Overview
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Product nameAnti-Glucocorticoid Receptor antibody [EPR19621]
See all Glucocorticoid Receptor primary antibodies -
DescriptionRabbit monoclonal [EPR19621] to Glucocorticoid Receptor
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Mouse Glucocorticoid Receptor aa 100-400. The exact sequence is proprietary.
Database link: P06537 -
Positive control
- WB: Human fetal heart and fetal kidney lysates; HeLa, HEK-293 and A431 whole cell lysates; HEK-293 whole cell lysate transfected with human Glucocorticoid Receptor with GFP-Myc tag. IHC-P: Human glioma and cervix carcinoma tissues; Mouse liver tissue; Rat hippocampus tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR19621
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 86, 83 kDa (predicted molecular weight: 86 kDa). | |
| IHC-P | 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/500. | |
| Flow Cyt | 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
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Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
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Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Post-translational
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Entrez Gene: 24413 Rat
- Omim: 138040 Human
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- SwissProt: P06536 Rat
- Unigene: 122926 Human
see all -
Alternative names
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
Images
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![Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266028-wb-1.jpg)
Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)All lanes : Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127) at 1/2000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 83,86 kDa (why is the actual band size different from the predicted?)Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 30 seconds; Lane 2: 15 seconds.
This antibody may recognize eight isoforms. The predicted MW are from 61KDa to 86KDa in human, respectively.
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![Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266026-wb-2.jpg)
Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)All lanes : Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 86 kDa
Observed band size: 83,86 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This antibody may recognize eight isoforms. The predicted MW are from 61KDa to 86KDa in human, respectively.
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![Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266025-wb-3.jpg)
Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)All lanes : Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127) at 1/20000 dilution
Lane 1 : Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with human Glucocorticoid Receptor with GFP-Myc tag
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 112 kDa (why is the actual band size different from the predicted?)Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 0.5 second.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266024-IHC-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Glucocorticoid Receptor with ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the Human glioma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266023-IHC-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Glucocorticoid Receptor with ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the cervix carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266022-IHC-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glucocorticoid Receptor with ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on hepatocytes of the mouse liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266021-IHC-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glucocorticoid Receptor with ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat hippocampus is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266020-IF-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glucocorticoid Receptor with ab183127 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
The results show signal translocation after dexamethasone (100 nM for 2 hours) treatment on Hela cells. PMID: 24291004.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab183127 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
![Flow Cytometry - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)](http://a.abcam.com/ps/products/183/ab183127/Images/ab183127-266019-FC-1.jpg)
Flow Cytometry - Anti-Glucocorticoid Receptor antibody [EPR19621] (ab183127)Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glucocorticoid Receptor with ab183127 at 1/500 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
Protocols
References
ab183127 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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