Overview

  • Product nameGlucocorticoid Receptor Kit
    See all Glucocorticoid Receptor kits
  • Sample type
    Nuclear Extracts
  • Assay typeSemi-quantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: all Mammals
  • Product overview

    Glucocorticoid receptor (GR) belongs to the nuclear receptor superfamily of structually related ligand-inducible transcription factors. GR acts as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors.

    Abcam's Glucocorticoid Receptor Kit can be used in gel mobility shift assays to investigate the role of GR in different tissues or the role of GRE under specific conditions such as hormone stimulation.

    Each kit contains enough protein for approximately 50 gel mobility shift reactions. For supershift assays, we recommend the use of Rabbit polyclonal anti- Glucocorticoid Receptor antibody (ab3671).

  • Notes

    Vial A contains recombinant human glucorticoid receptor (full length) that was expressed in an insect cell expression system and enriched by ammonium sulfate precipitation followed by dialysis in KED buffer with 20% glycerol.

     

    Vial B contains a dsDNA oligonucleotide representing the human tyrosine amino transferase gene response element. See protocol for specific sequence.

Properties

  • FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Alternative names
    • GCCR
    • GCR
    • GCR_HUMAN
    • GCRST
    • glucocorticoid nuclear receptor variant 1
    • Glucocorticoid receptor
    • GR
    • GRL
    • Grl1
    • nr3c1
    • Nuclear receptor subfamily 3 group C member 1
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab3582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration.

Before use, oligonucleotide should be labeled with

References for Glucocorticoid Receptor Kit (ab3582)

ab3582 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
I have received the following information from the lab:
Vial A contains 50 ul of recombinant human glucocorticoid receptor at 3.5 mg/ml. So you will receive 175 ug recombinant protein.
Vial B contains 20 ul o...

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Thank you for contacting us.

Vial A in ab3582 is 50ul of recombinant human glucocorticoid receptor at 3.5mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

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Thank you for your enquiry today. With regards to your technical question, the percent purity for ab3582 is ~95-96 percent. Additionally, I have provided some Gel Shift troubleshooting tips below. Troubleshooting for Gel Shift using ab3582: ...

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Thank you for your enquiry. Ab3582 consists of recombinant human glucocorticoid receptor (full length) that was expressed in an insect cell expression system and enriched by ammonium sulfate precipitation followed by dialysis in KED buffer with 20%...

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Thank you for your enquiry. The oligonucleotide vial contains the tyrosine aminotransferase GRE (tatGRE). If you require further clarification, please contact us again and we will gladly assist you.

Thank you for your enquiry. ab3582 is an oligonucleotide double stranded DNA representing the human tyrosine amino transferase gene response element with the following sequences: 5’...CTA GGC TGT ACA GGA TGT TCT GCC TAG...3’ 3’...GAT CCG ACA TGT CCT A...

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This is true. If you have any further questions, please contact us again.

I beleive it contains both isoforms. If there is any other way we can help you, please let us know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"