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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Glucocorticoid Receptor, phosphorylated at S226 and phosphorylated at S226.
(Peptide available as ab109640.)
Our Abpromise guarantee covers the use of ab93104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).
The glucocorticoid receptor contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab93104 has not yet been referenced specifically in any publications.