Anti-Glucokinase antibody [3E3] (ab117856)

Overview

  • Product nameAnti-Glucokinase antibody [3E3]
    See all Glucokinase primary antibodies
  • Description
    Mouse monoclonal [3E3] to Glucokinase
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Glucokinase.
    Database link: P35557

  • Positive control
    • HEK293T transfected lysate COS7 transfected cells Human breast, breast carcinoma, kidney, kidney carcinoma, lung, lung carcinoma, pancreas, thyroid carcinoma or prostrate carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab117856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 52 kDa.
IHC-P 1/50.
Flow Cyt 1/500. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionCatalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • Tissue specificityIsoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • Involvement in diseaseDefects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • Sequence similaritiesBelongs to the hexokinase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all

Anti-Glucokinase antibody [3E3] images

  • All lanes : Anti-Glucokinase antibody [3E3] (ab117856) at 1/500 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY GLK cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200472)
  • Immunofluorescence image using ab117856 at a 1/100 dilution, staining COS7 transfected cells.
  • ab117856, at a 1/50 dilution, staining Human Glucokinase in breast, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in breast adenocarcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in kidney, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in kidney carcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in lung , using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in lung carcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in pancreas, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in thyroid carcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

  • ab117856, at a 1/50 dilution, staining Human Glucokinase in prostrate carcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

References for Anti-Glucokinase antibody [3E3] (ab117856)

ab117856 has not yet been referenced specifically in any publications.

Product Wall

The immunogen that generated ab117856 was protein expressed in 293T cells transfected with Human GLK (NM_000162) expression vector. We do not have that particular recombinant protein for sale but we do have recombinant human GLK that will be similar, a...

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